组织蛋白酶B的免疫组织化学研究。对人类肺癌的预后意义。
Immunohistochemical study of cathepsin B. Prognostic significance in human lung cancer.
作者信息
Sukoh N, Abe S, Ogura S, Isobe H, Takekawa H, Inoue K, Kawakami Y
机构信息
First Department of Medicine, School of Medicine, Hokkaido University Hospital, Sapporo, Japan.
出版信息
Cancer. 1994 Jul 1;74(1):46-51. doi: 10.1002/1097-0142(19940701)74:1<46::aid-cncr2820740109>3.0.co;2-g.
BACKGROUND
The concentration of cysteine proteinase cathepsin B has been shown to be elevated in association with malignancy or metastatic potential of human and rodent tumors, but its prognostic value for human lung cancer remains undetermined.
METHODS
Using a polyclonal antibody, immunohistochemical analyses of cathepsin B were performed on paraffin embedded sections of tumors obtained surgically from 108 patients with non-small cell lung cancer (49 squamous cell carcinomas, 59 adenocarcinomas). The immunohistochemical expressions of cathepsin B in the tumors were compared with patient survival.
RESULTS
Higher grade expression of cathepsin B was associated significantly with shorter survival in non-small cell lung cancer (P < 0.01), in squamous cell carcinoma (P < 0.05), and in adenocarcinoma (P < 0.01). A similar result also was seen in Stage I non-small cell lung cancer (P < 0.05).
CONCLUSIONS
The authors concluded that the immunohistochemical staining pattern of cathepsin B may be a useful predictor of survival for human lung cancer.
背景
半胱氨酸蛋白酶组织蛋白酶B的浓度已被证明与人类和啮齿动物肿瘤的恶性或转移潜能相关，但其对人类肺癌的预后价值仍未确定。
方法
使用多克隆抗体，对108例非小细胞肺癌患者（49例鳞状细胞癌，59例腺癌）手术切除的肿瘤石蜡包埋切片进行组织蛋白酶B的免疫组化分析。将肿瘤中组织蛋白酶B的免疫组化表达与患者生存率进行比较。
结果
组织蛋白酶B的高分级表达与非小细胞肺癌患者的较短生存期显著相关（P<0.01），在鳞状细胞癌中（P<0.05），以及在腺癌中（P<0.01）。在I期非小细胞肺癌中也观察到类似结果（P<0.05）。
结论
作者得出结论，组织蛋白酶B的免疫组化染色模式可能是人类肺癌生存的有用预测指标。
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