Ortega E, Barriga C, Rodríguez A B
Department of Animal Physiology, Faculty of Sciences, University of Extremadura, Badajoz, Spain.
Comp Immunol Microbiol Infect Dis. 1994 Feb;17(1):77-84. doi: 10.1016/0147-9571(94)90009-4.
An investigation was made of the effects of a short and acute exposure to cigarette smoke on the capacity of alveolar macrophages from mice to carry out all stages of the phagocytic process. Cigarettes were commercial 80 mm filter cigarettes that contained 17 mg of tar and 1.1 mg of nicotine per cigarette. The acute exposure of each animal was with one cigarette for 15 min (until the complete consumption of the cigarette) in a box-shaped plastic chamber, 7732 cm3 vol and 450 cm2 floor surface, with a 3 cm dia airhole. Animals were sacrificed immediately after the exposure to the smoke. The results showed no differences either in the adherence or in the chemotaxis capacities between alveolar macrophages from control mice and from mice exposed to cigarette smoke. However, there was a significant decline both in attachment capacity and in ingestion capacity for Candida albicans. The reason for this was a decline in the number of macrophages with phagocytic capacity (percent of phagocytosis) and in the number of C. albicans phagocytized per cell (phagocytic efficiency). The conclusion is that a short, acute exposure to a smoke-filled atmosphere induced a decrease in the phagocytic function of alveolar macrophages.
研究了短期急性接触香烟烟雾对小鼠肺泡巨噬细胞进行吞噬过程各阶段能力的影响。香烟为市售80毫米过滤嘴香烟，每支含17毫克焦油和1.1毫克尼古丁。每只动物在一个容积为7732立方厘米、底面面积为450平方厘米、有一个直径3厘米气孔的箱形塑料室内，用一支香烟急性暴露15分钟（直至香烟完全燃尽）。暴露于烟雾后立即处死动物。结果显示，对照小鼠和暴露于香烟烟雾的小鼠的肺泡巨噬细胞在黏附能力或趋化能力方面均无差异。然而，白色念珠菌的附着能力和摄取能力均显著下降。原因是具有吞噬能力的巨噬细胞数量（吞噬百分比）以及每个细胞吞噬的白色念珠菌数量（吞噬效率）均下降。结论是，短期急性暴露于充满烟雾的环境会导致肺泡巨噬细胞吞噬功能下降。
