Le Gal F, Gasqui P, Renard J P
INRA, Unité de Biologie du Dévelopment, Joury-en-Josas, France.
Cryobiology. 1994 Apr;31(2):154-70. doi: 10.1006/cryo.1994.1019.
This study shows that immature and mature goat oocytes respond differently to hyperosmotic stress; when exposed to a 1.5 M propanediol solution, immature oocytes manifest a higher osmotic stress than do mature oocytes. This is the consequence of both higher water permeability (133.9 +/- 15.2 vs 82.4 +/- 4.4 x 10(-3) cm/min) and lower propanediol permeability (0.87 +/- 0.03 vs 1.20 +/- 0.03 x 10(-3) cm/min at 20 degrees C) in the immature than in the mature stage. The difference of osmotic behavior between these two types of oocytes is abolished following exposure to cytochalasin D, a drug known to modify the cellular microfilament network. This result suggests differences in actin organization between the two types of oocyte, probably at the cortical level. Calculated values of the intracellular concentration of propanediol as a function of time of exposure show that propanediol rapidly permeates both types of oocyte and that the kinetics of intracellular concentration are lowered by cytochalasin treatment.
本研究表明，未成熟和成熟的山羊卵母细胞对高渗应激的反应不同；当暴露于1.5 M丙二醇溶液时，未成熟卵母细胞表现出比成熟卵母细胞更高的渗透压应激。这是由于未成熟阶段比成熟阶段具有更高的水渗透性（133.9±15.2对82.4±4.4×10⁻³ cm/min）和更低的丙二醇渗透性（20℃时为0.87±0.03对1.20±0.03×10⁻³ cm/min）。在暴露于细胞松弛素D（一种已知可改变细胞微丝网络的药物）后，这两种类型卵母细胞之间的渗透行为差异消失。该结果表明两种类型卵母细胞之间肌动蛋白组织存在差异，可能在皮质水平。丙二醇细胞内浓度随暴露时间的计算值表明，丙二醇能迅速渗透到两种类型的卵母细胞中，并且细胞松弛素处理会降低细胞内浓度的动力学。
