Bulletti C, Galassi A, Parmeggiani R, Polli V, Alfieri S, Labate A M, Flamigni C
Department of Obstetrics and Gynecology, University of Bologna, Italy.
Fertil Steril. 1994 Jul;62(1):96-102.
To determine endometrial changes throughout the menstrual cycle.
Flow cytometric analysis of endometrial cells versus chronological dating.
Women volunteers with a normal menstrual cycle participated in this study that was done in an academic research environment.
Two hundred thirty regular menstruating women with adequate luteal phase underwent endometrial biopsy at different days of their menstrual cycle; 138 biopsies were analyzed both histologically and by flow cytometry.
Percentage of biopsies correctly classified in terms of chronological dating.
Flow cytometry correctly classified the chronological day of biopsies in 59% of cases, the proliferative versus secretory days in 91% of cases, and the early, mid, and late secretory phases of the menstrual cycle were correctly predicted in 86% of cases.
Flow cytometry is a good method to determine growth and differentiation of the endometrium during the menstrual cycle. Detection of phases of the whole endometrial cell population offer information on the biological adequacy of endometrial response to the biochemical environment.
确定整个月经周期中子宫内膜的变化。
子宫内膜细胞的流式细胞术分析与按时间顺序的日期测定。
月经周期正常的女性志愿者参与了这项在学术研究环境中进行的研究。
230名黄体期正常的规律月经女性在月经周期的不同时间接受了子宫内膜活检；对138份活检组织进行了组织学和流式细胞术分析。
按时间顺序日期正确分类的活检组织百分比。
流式细胞术在59%的病例中正确分类了活检组织的时间顺序日期，在91%的病例中正确区分了增殖期与分泌期，在86%的病例中正确预测了月经周期的早、中、晚分泌期。
流式细胞术是确定月经周期中子宫内膜生长和分化的良好方法。检测整个子宫内膜细胞群体的阶段可提供有关子宫内膜对生化环境反应的生物学适宜性的信息。
