Hoffmann A A, Clancy D J, Merton E
Department of Genetics and Human Variation, La Trobe University, Bundoora, Victoria, Australia.
Genetics. 1994 Mar;136(3):993-9. doi: 10.1093/genetics/136.3.993.
In Drosophila melanogaster, weak incompatibility in crosses between infected and uninfected strains is associated with a Wolbachia microorganism. Crosses between infected males and uninfected females show a reduction (15-30%) in egg hatch. Progeny tests indicated that the infection is widespread in Australian D. melanogaster populations and that populations are polymorphic for the presence of the infection. The infection status of 266 lines from 12 populations along the eastern coast of Australia was determined by 4',6-diamidino-2-phenylindole (DAPI) staining of embryos. All populations contained both infected and uninfected flies. Infection frequencies varied between populations but there was no discernible geographical pattern. Laboratory experiments indicated that the infection was not associated with a reduction in fecundity as in Drosophila simulans. Incompatibility levels could not be increased by laboratory selection on isofemale lines. Factors contributing to the persistence of the infection in D. melanogaster populations are discussed.
在黑腹果蝇中，感染菌株与未感染菌株杂交时的弱不亲和性与一种沃尔巴克氏体微生物有关。感染的雄性与未感染的雌性杂交后，卵的孵化率会降低（15%-30%）。子代测试表明，这种感染在澳大利亚的黑腹果蝇种群中广泛存在，并且种群对于感染的存在具有多态性。通过对澳大利亚东海岸12个种群的266个品系的胚胎进行4',6-二脒基-2-苯基吲哚（DAPI）染色，确定了其感染状态。所有种群都同时含有感染和未感染的果蝇。感染频率在不同种群间有所差异，但没有明显的地理分布模式。实验室实验表明，这种感染与拟果蝇不同，它不会导致繁殖力下降。通过对同雌系进行实验室选择，无法提高不亲和水平。文中讨论了导致这种感染在黑腹果蝇种群中持续存在的因素。
