Kern M, Mondon C, Butte J C, Azhar S
Department of Medicine, Stanford University School of Medicine, California.
Horm Metab Res. 1994 Mar;26(3):129-32. doi: 10.1055/s-2007-1000792.
In this study we compared insulin binding activity, insulin receptor tyrosine kinase activity, and GLUT 4 protein content in six muscles from LA/N-cp rats and their lean controls. LA/N-cp rats had an approximate 20-fold increase in insulin concentration (837 +/- 113 vs 40 +/- 1), associated with significant (p < 0.01) decreases in both insulin binding activity per mg muscle and in muscle GLUT 4 content. Maximum insulin tyrosine kinase activity was also lower in muscle from LA/N-cp rats, but no difference was noted when tyrosine kinase activity was expressed per receptor. These data indicate that there are at least two defects in the insulin action cascade in muscle from LA/N-cp rats that contribute to the insulin resistance in these animals.
在本研究中，我们比较了LA/N-cp大鼠及其瘦素对照大鼠的六种肌肉中的胰岛素结合活性、胰岛素受体酪氨酸激酶活性和GLUT 4蛋白含量。LA/N-cp大鼠的胰岛素浓度增加了约20倍（837±113对40±1），同时每毫克肌肉的胰岛素结合活性和肌肉GLUT 4含量均显著降低（p<0.01）。LA/N-cp大鼠肌肉中的最大胰岛素酪氨酸激酶活性也较低，但当按每个受体表达酪氨酸激酶活性时，未发现差异。这些数据表明，LA/N-cp大鼠肌肉中的胰岛素作用级联至少存在两个缺陷，这导致了这些动物的胰岛素抵抗。
