Gonoi T, Mizuno N, Inagaki N, Kuromi H, Seino Y, Miyazaki J, Seino S
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan.
J Biol Chem. 1994 Jun 24;269(25):16989-92.
We report that a non-neuronal cell line, MIN6, derived from insulin-secreting pancreatic beta-cells, naturally expresses functional ionotropic glutamate receptors. Electrophysiological recordings show that kainate, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and N-methyl-D-aspartate (NMDA) depolarize single MIN6 cells and evoke inward ionic currents. These agents also increase the intracellular calcium concentration in MIN6 cells. Furthermore, insulin secretion from MIN6 cells is stimulated by kainate, AMPA, and NMDA. The presence of AMPA/kainate and NMDA receptor subtypes is confirmed by reverse transcriptase-polymerase chain reaction. These results demonstrate that ionotropic glutamate receptors with properties similar to those in neuronal cells are expressed in a non-neuronal cell line, MIN6. Thus, MIN6 provides a useful and valuable model system for biochemical, pharmacological, and physiological studies of ionotropic glutamate receptors.
我们报告称，一种源自胰岛素分泌性胰腺β细胞的非神经元细胞系MIN6天然表达功能性离子型谷氨酸受体。电生理记录显示，海人酸、α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）和N-甲基-D-天冬氨酸（NMDA）可使单个MIN6细胞去极化并引发内向离子电流。这些试剂还会增加MIN6细胞内的钙浓度。此外，海人酸、AMPA和NMDA可刺激MIN6细胞分泌胰岛素。逆转录聚合酶链反应证实了AMPA/海人酸和NMDA受体亚型的存在。这些结果表明，具有与神经元细胞中类似特性的离子型谷氨酸受体在非神经元细胞系MIN6中表达。因此，MIN6为离子型谷氨酸受体的生化、药理学和生理学研究提供了一个有用且有价值的模型系统。
