Iio K, Minoura N, Aiba S, Nagura M, Kodama M
National Institute of Materials and Chemical Research, Ibaraki, Japan.
J Biomed Mater Res. 1994 Apr;28(4):459-62. doi: 10.1002/jbm.820280408.
A cationic, high-water-content hydrogel membrane composed of poly(vinyl alcohol) (PVA) and poly(ally-biguanido-co-allylamine) hydrochloride (PAB) with positively charged biguanido groups that resemble arginine residues was developed. The PAB was prepared by reacting poly(allylamine) hydrochloride (PAA) with guanyl-O-methyl isourea. PAB/PVA hydrogel membranes were prepared by repeated freezing and thawing. For comparison, hydrogel membranes composed of PAA and PVA were also prepared. The interaction between these hydrogel membranes and mouse fibroblast (L929) was studied by a cell culture method. The PAB hydrogel blend had a relatively low percentage of initial cell attachment. The cell growth on the PAB hydrogel membranes showed a maximum at 5 mol % PAB content that was as high as commercially available plastic films. However, cells on hydrogel membranes with 50 mol % PAB content and 0 mol % PAB content (only PVA) did not seem to grow; neither did the 5/95 PAA/PVA membranes. Water contact angles of hydrogel membranes did not vary with the PAB content. Morphology of the cell attachment was observed by SEM. On the PAB blend hydrogel surfaces, cells were not spindle-shaped and monolayers, but rather cells aggregated in spherical clusters.
制备了一种由聚乙烯醇（PVA）和聚（烯丙基双胍 - 共 - 烯丙胺）盐酸盐（PAB）组成的阳离子高含水量水凝胶膜，其中带正电荷的双胍基团类似于精氨酸残基。PAB是通过使盐酸聚烯丙胺（PAA）与胍基 - O - 甲基异脲反应制备的。PAB/PVA水凝胶膜通过反复冻融制备。为了进行比较，还制备了由PAA和PVA组成的水凝胶膜。通过细胞培养方法研究了这些水凝胶膜与小鼠成纤维细胞（L929）之间的相互作用。PAB水凝胶共混物的初始细胞附着百分比相对较低。在PAB水凝胶膜上的细胞生长在PAB含量为5摩尔％时达到最大值，与市售塑料薄膜一样高。然而，PAB含量为50摩尔％和0摩尔％（仅PVA）的水凝胶膜上的细胞似乎没有生长；5/95 PAA/PVA膜上的细胞也没有生长。水凝胶膜的水接触角不随PAB含量而变化。通过扫描电子显微镜观察细胞附着的形态。在PAB共混水凝胶表面上，细胞不是纺锤形且不是单层的，而是聚集成球形簇。
