Hettler D, Andry M C, Levy M C
Laboratoire de Pharmacotechnie, URA/CNRS 492, Faculté de Pharmacie, Université de Reims Champagne-Ardenne, France.
J Microencapsul. 1994 Mar-Apr;11(2):213-24. doi: 10.3109/02652049409040453.
Microcapsules were prepared from three proteins, namely human serum albumin (HSA), bovine fibrinogen and ovalbumin, by an interfacial crosslinking process using terephthaloylchloride. They were further treated with alkaline hydroxylamine in order to disrupt ester and anhydride bonds in the walls. All microcapsules survived the treatment. They exhibited a significant increase in size and became sensitive to trypsin. The hydroxylamine treatment also resulted in attachment of hydroxamic groups to the membrane, making the microcapsules capable of iron binding. These properties were evaluated after soaking microcapsules in a 140 mumol/l ferric solution and determination of iron in the supernantant. Lower amounts of iron were found to be complexed by HSA microcapsules (mean value: 29.3 mumol iron/g microcapsule dry weight) as compared with fibrinogen and ovalbumin microcapsules (43.7 and 44.9 mumol/g, respectively). Microcapsule chelating properties were further improved by esterification of the free carboxyl groups of the membrane with benzyl alcohol or ethanol using a carbodiimide, prior to the hydroxylamine treatment. Comparable values of iron binding were obtained from esterified and hydroxylamine-treated batches prepared from the three proteins (about 50 mumol iron/g).
通过使用对苯二甲酰氯的界面交联过程，由三种蛋白质，即人血清白蛋白（HSA）、牛纤维蛋白原和卵清蛋白制备微胶囊。它们进一步用碱性羟胺处理，以破坏壁中的酯键和酸酐键。所有微胶囊在处理后都存活下来。它们的尺寸显著增加，并对胰蛋白酶变得敏感。羟胺处理还导致异羟肟酸基团附着在膜上，使微胶囊能够结合铁。在将微胶囊浸泡在140μmol/L的铁溶液中并测定上清液中的铁之后，对这些性质进行了评估。与纤维蛋白原和卵清蛋白微胶囊（分别为43.7和44.9μmol/g）相比，发现HSA微胶囊结合的铁量较低（平均值：29.3μmol铁/g微胶囊干重）。在羟胺处理之前，通过使用碳二亚胺用苄醇或乙醇将膜的游离羧基酯化，进一步改善了微胶囊的螯合性能。由这三种蛋白质制备的酯化和羟胺处理批次获得了相当的铁结合值（约50μmol铁/g）。
