Tsukimori K, Maeda H, Shingu M, Koyanagi T, Nobunaga M, Nakano H
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Hum Hypertens. 1994 Mar;8(3):177-80.
To clarify the pathophysiological changes of pre-eclampsia, we investigated the injurious effect of sera from women with pre-eclampsia on cultured endothelial cells, vascular smooth muscle cells and fibroblasts. We obtained serum samples from 32 Japanese women, including 10 healthy nonpregnant women, 12 normal pregnant women and 10 women with pre-eclampsia. Cell injury was measured by the release of radiolabelled chromium from the cells into the culture medium. The mean values +/- SD of percentage chromium release from endothelial cells in normal nonpregnant, normal pregnant and pre-eclamptic subjects were 8.5 +/- 2.4, 8.8 +/- 2.1 and 19.7 +/- 3.6%, respectively. Sera from women with pre-eclampsia demonstrated significantly greater endothelial cell injury than did sera from normal pregnant and nonpregnant subjects. However, normal pregnant and pre-eclamptic subjects did not differ with respect to both vascular smooth muscle cell injury and fibroblast injury. These results indicate that a serum cytotoxic to endothelial cells is present in pre-eclampsia and that this activity has a cellular specificity for endothelial cells.
为阐明子痫前期的病理生理变化，我们研究了子痫前期患者血清对培养的内皮细胞、血管平滑肌细胞和成纤维细胞的损伤作用。我们从32名日本女性中获取了血清样本，其中包括10名健康未孕女性、12名正常孕妇和10名子痫前期患者。通过测量放射性标记的铬从细胞释放到培养基中的量来检测细胞损伤。正常未孕、正常妊娠和子痫前期受试者的内皮细胞铬释放百分比的平均值±标准差分别为8.5±2.4%、8.8±2.1%和19.7±3.6%。子痫前期患者的血清对内皮细胞的损伤明显大于正常妊娠和未孕受试者的血清。然而，正常妊娠和子痫前期受试者在血管平滑肌细胞损伤和成纤维细胞损伤方面没有差异。这些结果表明，子痫前期存在对内皮细胞具有细胞毒性的血清，且这种活性对内皮细胞具有细胞特异性。
