Robinson E S, VandeBerg J L
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78228-0147.
Lab Anim Sci. 1994 Feb;44(1):63-8.
Procedures have been developed for collecting blood samples from the laboratory opossum (Monodelphis domestica) and for conducting survival surgery on adults. Blood samples up to 1 ml were collected routinely by cardiac puncture of animals anesthetized by inhalation of methoxyflurane or halothane with minimal risk; however, halothane required more careful monitoring. Surgical procedures such as partial hepatectomy unilateral hysterectomy, and tail wound repair were safely accomplished on animals anesthetized with methoxyflurane. Other surgical procedures including enucleation and intra-abdominal vasectomy were carried out successfully with an inhalation/injection (pentobarbital-atropine-ketamine) protocol that was successful also for recipient surrogate females in embryo transfer experiments.
已经开发出从实验负鼠（家短尾负鼠）采集血样以及对成年负鼠进行存活手术的程序。通过对吸入甲氧氟烷或氟烷麻醉的动物进行心脏穿刺，常规采集多达1毫升的血样，风险极小；然而，氟烷需要更仔细的监测。在用甲氧氟烷麻醉的动物身上安全地完成了诸如部分肝切除术、单侧子宫切除术和尾部伤口修复等外科手术。其他外科手术，包括眼球摘除术和腹腔内输精管切除术，采用吸入/注射（戊巴比妥-阿托品-氯胺酮）方案成功实施，该方案在胚胎移植实验中对受体代孕雌性动物也同样成功。
