Chen H L, Kamath R, Pace J L, Russell S W, Hunt J S
Department of Anatomy, University of Kansas Medical Center, Kansas City 66160-7400.
Placenta. 1994 Feb-Mar;15(2):109-21. doi: 10.1016/s0143-4004(05)80448-5.
In order to evaluate the potential of placental cells to bind the multifunctional cytokine, interferon-gamma (IFN-gamma), tissues collected from pregnant Swiss mice were analysed for IFN-gamma receptor (IFN-gamma R) mRNA and protein. Northern blot hybridization studies indicated that the relative abundance of IFN-gamma R mRNA increased as gestation progressed to term. Analysis by in situ hybridization revealed that trophoblast cells first contained high steady state levels of IFN-gamma R mRNA at g.d. 12. At g.d. 12 and 14, transcription was restricted to cells in the spongiotrophoblast region and nests of similar cells in the labyrinthine region. These cells also contained immunoreactive IFN-gamma R protein. By g.d. 18, IFN-gamma R mRNA was clearly detectable in large spongiotrophoblast cells and labyrinthine trophoblast. IFN-gamma R mRNA was low to absent in giant trophoblast cells at all stages of gestation. Specific mRNA was present in parietal and visceral yolk sac cells by g.d. 14. Thus, expression of the IFN-gamma R gene in mouse placental cells is influenced by stage of gestation, cell lineage and state of differentiation. Whether or not these cells respond vigorously to IFN-gamma with induction of antiviral proteins, increased MHC class I antigens and growth modulation may therefore be determined by their expression of specific receptors for this pluripotent cytokine.
为了评估胎盘细胞结合多功能细胞因子γ干扰素（IFN-γ）的潜力，对从怀孕的瑞士小鼠收集的组织进行了IFN-γ受体（IFN-γR）mRNA和蛋白分析。Northern印迹杂交研究表明，随着妊娠进展至足月，IFN-γR mRNA的相对丰度增加。原位杂交分析显示，滋养层细胞在妊娠第12天首次含有高水平的IFN-γR mRNA稳态。在妊娠第12天和14天，转录仅限于海绵滋养层区域的细胞以及迷路区域中类似细胞的巢。这些细胞也含有免疫反应性IFN-γR蛋白。到妊娠第18天，在大型海绵滋养层细胞和迷路滋养层中可清楚检测到IFN-γR mRNA。在妊娠各阶段，巨型滋养层细胞中IFN-γR mRNA含量低或无。到妊娠第14天，壁层和脏层卵黄囊细胞中存在特异性mRNA。因此，小鼠胎盘细胞中IFN-γR基因的表达受妊娠阶段、细胞谱系和分化状态的影响。因此，这些细胞是否通过诱导抗病毒蛋白、增加MHC I类抗原和调节生长而对IFN-γ产生强烈反应，可能取决于它们对这种多能细胞因子特异性受体的表达。
