Sugawara N, Katakura M, Li D, Sugawara C, Miyake H
Department of Public Health, School of Medicine, Sapporo Medical University, Japan.
Res Commun Chem Pathol Pharmacol. 1994 Mar;83(3):349-58.
Liver slices from Wistar and Long-Evans Cinnamon (LEC) rats were incubated while open to the atmosphere to assess the liver function in LEC rats. Leakages of glutamic-oxaloacetic transaminase (GOT) and lactic dehydrogenase (LDH) into the medium were significantly lower in the LEC rat than in the Wistar rat. Furthermore, no pronounced enhancement of the concentration of thiobarbituric acid-reactive substances (TBARS) was found in the LEC rat. Hepatic Cu and Cu-metallothionein (Cu-MT) concentrations were 355.0 +/- 18.7 micrograms/g liver and 2559 +/- 181 micrograms/g protein in the LEC rats, whereas Wistar rats showed 4.1 +/- 0.1 Cu microgram/g liver accompanied by 16 +/- 4 micrograms/g protein of MT. The decrease of intrahepatic Cu-MT in LEC rats was stimulated by incubation with Fenitrilotriacetate (Fe-NTA). There was a direct correlation between the enhancement of TBARS and disappearance of Cu-MT. Our results suggest that hepatic Cu-MT in LEC rats protects against liver injury stimulated by oxidative stress.
将Wistar大鼠和长 Evans 肉桂（LEC）大鼠的肝切片置于大气环境中孵育，以评估LEC大鼠的肝功能。与Wistar大鼠相比，LEC大鼠的谷氨酸草酰乙酸转氨酶（GOT）和乳酸脱氢酶（LDH）向培养基中的渗漏显著降低。此外，在LEC大鼠中未发现硫代巴比妥酸反应性物质（TBARS）浓度有明显升高。LEC大鼠肝脏中铜（Cu）和铜金属硫蛋白（Cu-MT）的浓度分别为355.0±18.7微克/克肝脏和2559±181微克/克蛋白质，而Wistar大鼠肝脏中铜含量为4.1±0.1微克/克，金属硫蛋白（MT）含量为16±4微克/克蛋白质。用亚硝基三乙酸铁（Fe-NTA）孵育可刺激LEC大鼠肝内Cu-MT含量降低。TBARS的升高与Cu-MT的消失之间存在直接相关性。我们的结果表明，LEC大鼠肝脏中的Cu-MT可保护肝脏免受氧化应激刺激引起的损伤。
