Silins S L, Sculley T B
Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia 4029.
Virology. 1994 Jul;202(1):16-24. doi: 10.1006/viro.1994.1317.
The growth transformation of human B cells by Epstein-Barr virus (EBV) is controlled by the coordinate expression of 10 latent viral genes. This transforming capacity is believed to be fundamental to the involvement of the virus in human malignancies of B cell origin. EBV-negative Burkitt's lymphoma (dG75) clones stably expressing one of these EBV-coded antigens, EBNA-4, have been established using an episomal-based plasmid. EBNA-4 expression was found to upregulate the cytoskeletal protein vimentin as well as surface expression of the activation antigen CD40. In addition, the presence of EBNA-4 resulted in downregulation of the Burkitt's lymphoma-associated antigen (BLA/CD77). These studies show for the first time that EBNA-4 modulates the expression of several cellular genes implicated in cell-growth transformation.
爱泼斯坦-巴尔病毒（EBV）对人类B细胞的生长转化由10种潜伏病毒基因的协同表达所控制。这种转化能力被认为是该病毒参与B细胞起源的人类恶性肿瘤的关键因素。利用基于游离型质粒已建立了稳定表达这些EBV编码抗原之一EBNA-4的EBV阴性伯基特淋巴瘤（dG75）克隆。研究发现，EBNA-4的表达上调细胞骨架蛋白波形蛋白以及活化抗原CD40的表面表达。此外，EBNA-4的存在导致伯基特淋巴瘤相关抗原（BLA/CD77）表达下调。这些研究首次表明，EBNA-4可调节与细胞生长转化相关的几种细胞基因的表达。
