Faisst S, Perros M, Deleu L, Spruyt N, Rommelaere J
Institut Pasteur de Lille, Molecular Oncology Unit, CNRS URA 1160, Lille, France.
Virology. 1994 Jul;202(1):466-70. doi: 10.1006/viro.1994.1363.
The early promoter (P4) of the autonomous parvovirus minute virus of mice (prototype strain) directs the expression of the transcription unit coding for the nonstructural proteins NS1 and NS2. Although proximal promoter elements (GC and TATA boxes) are essential for P4 activity in vivo, additional upstream sequences appear to be required for optimal transcription. Therefore, associations of proteins with the upstream regulatory region of promoter P4 were studied in the rat fibroblast cell line FREJ4 by gel retardation and in vitro as well as in vivo footprinting assays. This led to the identification of at least four distinct upstream elements that interacted with cellular proteins. The functionality of these elements was supported by the reduced level of gene expression driven by corresponding linker-substitutive mutants of promoter P4.
小鼠自主细小病毒（原型株）的早期启动子（P4）指导编码非结构蛋白NS1和NS2的转录单位的表达。尽管近端启动子元件（GC盒和TATA盒）对于P4在体内的活性至关重要，但似乎还需要额外的上游序列来实现最佳转录。因此，通过凝胶阻滞以及体外和体内足迹分析，在大鼠成纤维细胞系FREJ4中研究了蛋白质与启动子P4上游调控区的关联。这导致鉴定出至少四个与细胞蛋白相互作用的不同上游元件。由启动子P4的相应接头取代突变体驱动的基因表达水平降低，支持了这些元件的功能。
