Lu Z H, Yang Y C, Shen S Y, Tan W Q
Department of Oncology, Wuhan General Hospital, China.
Zhongguo Yao Li Xue Bao. 1993 Nov;14 Suppl:S28-30.
A semi-solid cell culture technique was used to study the sensitivity of K562, HL-60, and Raji leukemic cell lines to the inhibitory effect of psoralen plus ultraviolet irradiation. Results indicated that: 1) the inhibition rate of K562, HL-60, and Raji cell lines were 86%, 35%, and 36%, respectively; and 2) K562, HL-60, and Raji cell lines were treated with psoralen (20 micrograms.ml-1) for 1 h, then irradiated with ultraviolet (1 J/cm2) for 10 min, none of the leukemic cell lines showed colony or cluster formation. These suggested that the cytocidal effect of psoralen plus ultraviolet might be useful to eradicate the residual leukemic cells in the bone marrow transplantation.
采用半固体细胞培养技术研究了补骨脂素加紫外线照射对K562、HL-60和Raji白血病细胞系的抑制作用敏感性。结果表明：1）K562、HL-60和Raji细胞系的抑制率分别为86%、35%和36%；2）用补骨脂素（20微克/毫升）处理K562、HL-60和Raji细胞系1小时，然后用紫外线（1焦耳/平方厘米）照射10分钟，所有白血病细胞系均未出现集落或细胞团形成。这些结果提示补骨脂素加紫外线的杀细胞作用可能有助于根除骨髓移植中残留的白血病细胞。
