作为复杂蛋白质生物反应器的猪乳腺。

The porcine mammary gland as a bioreactor for complex proteins.

作者信息

Morcöl T, Akers R M, Johnson J L, Williams B L, Gwazdauskas F C, Knight J W, Lubon H, Paleyanda R K, Drohan W N, Velander W H

机构信息

Department of Chemical Engineering, Virginia Polytechnic Institute and State University, Blacksburg 24061.

出版信息

Ann N Y Acad Sci. 1994 May 2;721:218-33. doi: 10.1111/j.1749-6632.1994.tb47394.x.

DOI:10.1111/j.1749-6632.1994.tb47394.x

PMID:8010672

Abstract

The similar biological activity of rhPC and hPC indicates that porcine mammary gland can perform many of the processing reactions necessary for recombinant synthesis of complex human proteins and produce them at levels suitable for industrial bioreactor applications. The health of the transgenic pigs appeared unaffected by the expression of high levels of the heterologous protein. We suggest that one of the advantages of using the mammary gland as a bioreactor appears to be the high cell density relative to that of cell culture.

摘要

重组猪蛋白C（rhPC）和人蛋白C（hPC）具有相似的生物学活性，这表明猪乳腺能够进行复杂人类蛋白质重组合成所需的许多加工反应，并以适合工业生物反应器应用的水平生产这些蛋白质。转基因猪的健康状况似乎未受高水平异源蛋白表达的影响。我们认为，将乳腺用作生物反应器的优势之一似乎是相对于细胞培养而言细胞密度较高。