Grohmann K, Baldwin E A, Buslig B S
USDA Citrus & Subtropical Products Laboratory, Winter Haven, FL 33881.
Appl Biochem Biotechnol. 1994 Spring;45-46:315-27. doi: 10.1007/BF02941808.
We extended our previous investigations of enzymatic hydrolysis of polysaccharides in orange peel by commercial cellulase and pectinase enzymes to higher, more practical concentrations of orange peel solids. High yields of saccharification could be maintained even at substrate concentrations as high as 22-23%, but the rates of solubilization and saccharification decreased 2-3-fold. We also tested the fermentability of these hydrolysates by the yeast Saccharomyces cerevisiae, which revealed the presence of inhibitory compounds. These compounds could be removed by the filtration of hydrolyzed peel. Successful fermentations of filtered hydrolysates were achieved after pH adjustment with calcium carbonate.
我们将之前关于用商业纤维素酶和果胶酶对橙皮中的多糖进行酶解的研究扩展到了更高、更实际浓度的橙皮固体。即使在底物浓度高达22%-23%时，仍可保持较高的糖化产率，但溶解和糖化速率降低了2-3倍。我们还测试了酿酒酵母对这些水解产物的发酵能力，结果表明存在抑制性化合物。这些化合物可通过过滤水解后的果皮去除。在用碳酸钙调节pH后，成功实现了对过滤后水解产物的发酵。
