牛卵泡抑素cDNA的克隆与组织表达

Cloning and tissue expression of bovine follistatin cDNA.

作者信息

Houde A, Lussier J G, Ethier J F, Gagnon C, Silversides D W

机构信息

Department of Anatomy and Physiology, Faculty of Veterinary Medicine, University of Montreal, St. Hyacinthe, Québec, Canada.

出版信息

Mol Reprod Dev. 1994 Apr;37(4):391-7. doi: 10.1002/mrd.1080370405.

DOI:10.1002/mrd.1080370405

PMID:8011323

Abstract

Bovine follistatin cDNA sequences were derived using a cloning strategy based entirely on reverse transcription and polymerase chain reaction amplification of RNA derived from bovine ovarian and testicular tissues. Complete bovine follistatin cDNA coding sequences are presented including 1,029 bases of open reading frame, the 5' translational start codon, and the 3' translational stop codon. Homologies of bovine follistatin cDNA with pig, human, rat, and partial sheep sequences are 94.3%, 92.4%, 89.9%, and 98.4% at the nucleic acid level and 98.3%, 97.1%, 95.6%, and 100% at the deduced amino acid level, respectively. Northern blot analysis on a survey of bovine reproductive tissues showed strongest expression in ovaries collected from superovulated cows and major RNA species at 2.8 Kb and 1.75 Kb.

摘要

牛卵泡抑素cDNA序列是采用一种完全基于逆转录和聚合酶链反应扩增来自牛卵巢和睾丸组织的RNA的克隆策略获得的。文中给出了完整的牛卵泡抑素cDNA编码序列，包括1029个碱基的开放阅读框、5'翻译起始密码子和3'翻译终止密码子。牛卵泡抑素cDNA与猪、人、大鼠和部分绵羊序列在核酸水平上的同源性分别为94.3%、92.4%、89.9%和98.4%，在推导氨基酸水平上的同源性分别为98.3%、97.1%、95.6%和100%。对牛生殖组织的Northern印迹分析表明，在超排奶牛卵巢中的表达最强，主要RNA种类为2.8 Kb和1.75 Kb。