Morré D J, Paulik M, Lawrence J L, Morré D M
Department of Medicinal Chemistry and Pharmacognosy, Purdue University, West Lafayette, IN 47907.
FEBS Lett. 1994 Jun 13;346(2-3):199-202. doi: 10.1016/0014-5793(94)00469-2.
Reduced pyridine nucleotide has been reported to enhance cell-free transfer of membrane material from a radiolabeled Golgi apparatus donor fraction from rat liver to an acceptor fraction consisting of inside-out plasma vesicles immobilized on nitrocellulose [(1992) Biochim. Biophys. Acta 1107, 131]. As part of a continuing effort to identify NADH-requiring enzymes in the Golgi apparatus which may be important to membrane trafficking, highly purified fractions of Golgi apparatus from rat liver were tested for their ability to oxidize NADH and the inhibition of the oxidation of NADH by brefeldin A. The isolated Golgi apparatus fractions were found to oxidize NADH with a specific activity comparable to that of the plasma membrane of rat liver. The activity was inhibited by brefeldin A and this inhibition was augmented by GDP. At near optimal concentrations of 7 microM brefeldin A and 1 microM GDP, the activity was > 90% inhibited. Brefeldin A inhibition of NADH oxidation by the Golgi apparatus was time-dependent and GDP appeared to accelerate the inhibition by brefeldin A.
据报道，还原型吡啶核苷酸可增强膜材料的无细胞转移，这种转移是从大鼠肝脏放射性标记的高尔基体供体组分转移到由固定在硝酸纤维素上的外翻血浆囊泡组成的受体组分[（1992年）《生物化学与生物物理学报》1107, 131]。作为持续努力确定高尔基体中可能对膜运输很重要的需NADH酶的一部分，对来自大鼠肝脏的高度纯化的高尔基体组分进行了测试，以检测它们氧化NADH的能力以及布雷菲德菌素A对NADH氧化的抑制作用。发现分离的高尔基体组分氧化NADH的比活性与大鼠肝脏的质膜相当。该活性受到布雷菲德菌素A的抑制，并且这种抑制作用因GDP而增强。在7 microM布雷菲德菌素A和1 microM GDP的接近最佳浓度下，活性受到> 90%的抑制。布雷菲德菌素A对高尔基体氧化NADH的抑制作用是时间依赖性的，并且GDP似乎加速了布雷菲德菌素A的抑制作用。
