Inoue M, Okayama A, Fujita M, Enomoto T, Sakata M, Tanizawa O, Ueshima H
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Int J Cancer. 1994 Jul 1;58(1):14-9. doi: 10.1002/ijc.2910580104.
Using immunohistochemical methods, we analyzed the association between nuclear p53 overexpression and various clinicopathological parameters in patients with endometrial cancers. Formalin-fixed and paraffin-embedded tissue sections from 139 cases of endometrial cancer (endometrioid type, 126; serous papillary type, 12; and clear-cell type, 1) were stained with anti-p53 monoclonal antibody (MAb) DO7. Overexpression of p53 was associated with high malignant potential, including extensive muscular invasion, advanced surgical stage, high histological grade, serous papillary type and a personal history of cancer. Lymph-node metastasis was also related to p53 overexpression with marginal significance. Survival curves determined by the Kaplan-Meier method and univariate analysis showed p53 overexpression to be associated with a poor outcome in endometrial cancer patients. However, multivariate analysis using the stepwise Cox proportional-hazard model showed that whereas lymph-node metastasis, a personal history of cancer and muscular invasion were related to poor survival rates, p53 overexpression was not. Consequently, p53 overexpression itself does not appear to be an independent prognostic factor in endometrial cancer, although a still larger sample of patient material would be required to assess this issue definitively.
我们采用免疫组化方法分析了子宫内膜癌患者中核p53过表达与各种临床病理参数之间的关联。用抗p53单克隆抗体（MAb）DO7对139例子宫内膜癌（子宫内膜样型126例、浆液性乳头状型12例、透明细胞型1例）的福尔马林固定石蜡包埋组织切片进行染色。p53过表达与高恶性潜能相关，包括广泛肌层浸润、手术分期晚、组织学分级高、浆液性乳头状型以及个人癌症史。淋巴结转移也与p53过表达相关，但差异无统计学意义。采用Kaplan-Meier法和单因素分析确定的生存曲线显示，p53过表达与子宫内膜癌患者的不良预后相关。然而，使用逐步Cox比例风险模型进行的多因素分析显示，虽然淋巴结转移、个人癌症史和肌层浸润与生存率低相关，但p53过表达并非如此。因此，尽管需要更大样本的患者材料来明确评估这个问题，但p53过表达本身似乎并不是子宫内膜癌的独立预后因素。
