用高效液相色谱法测定血浆和尿液中的羧苄青霉素差向异构体。

Determination of carbenicillin epimers in plasma and urine with high-performance liquid chromatography.

作者信息

Ishida M, Tsuda Y, Onuki Y, Itoh T, Shimada H, Yamada H

机构信息

Department of Pharmacokinetics and Biopharmaceutics, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.

出版信息

J Chromatogr. 1994 Jan 14;652(1):43-9. doi: 10.1016/0378-4347(93)e0378-4.

DOI:10.1016/0378-4347(93)e0378-4

PMID:8014226

Abstract

A high-performance liquid chromatographic method was developed for determining the concentrations of carbenicillin (CBPC) epimers in plasma and urine. Samples were prepared for HPLC analysis by solid-phase extraction and the concentrations of CBPC epimers were determined using reversed-phase HPLC with a mixture of 0.05 M ammonium acetate and methanol as a mobile phase. Baseline separation of the two epimers was observed for both plasma and urine samples with a detection limit of ca. 10 micrograms/ml. No peaks interfering with either of the CBPC epimers were observed on the HPLC chromatograms for blank plasma and urine. The presented method was used to determine the protein binding of CBPC epimers in vitro in human and rabbit plasma. The stereoselectivity of the binding of CBPC appeared to be reversed in human and rabbit plasma.

摘要

建立了一种高效液相色谱法，用于测定血浆和尿液中羧苄青霉素（CBPC）差向异构体的浓度。通过固相萃取制备样品用于HPLC分析，并使用以0.05 M醋酸铵和甲醇的混合物作为流动相的反相HPLC测定CBPC差向异构体的浓度。在血浆和尿液样品中均观察到两种差向异构体的基线分离，检测限约为10微克/毫升。在空白血浆和尿液的HPLC色谱图上未观察到干扰任何一种CBPC差向异构体的峰。所提出的方法用于体外测定人血浆和兔血浆中CBPC差向异构体的蛋白结合率。CBPC结合的立体选择性在人血浆和兔血浆中似乎是相反的。