Bose S K, Ortonne J P
Pasteur Hospital, University of Nice.
J Dermatol. 1994 Mar;21(3):152-9. doi: 10.1111/j.1346-8138.1994.tb01713.x.
It has been suggested that, in vitiligo, the structural abnormalities may not be restricted to melanocytes alone, but also to keratinocytes of involved and uninvolved skin. Focal gaps in the vitiligo epidermal basement membrane have been furthermore interpreted as evidence of extramelanocyte alterations. To understand the occurrence of the gaps we undertook a study to establish which cells are related to them. Immunofluorescence and immunoperoxidase techniques were used to visualize, in involved, uninvolved, and control skin, the basement membrane, melanocytes, and Merkel cells. Double staining was performed with fluorescein-coupled anti-collagen IV antibodies and rhodamin-coupled antibodies against Nicein (GB3), a hemidesmosome component. Propidium iodide staining was used to detect infiltrating lymphocytes. The basement membrane structure was always found to be intact and unaltered. Absence of staining in areas of uninvolved skin was related to the presence of Merkel cells, melanocytes, and infiltrating lymphocytes. Gaps in the epidermal basement membrane are thus due to lack of staining caused by the presence of immigrant cells.
有人提出，在白癜风中，结构异常可能不仅限于黑素细胞，还涉及受累皮肤和未受累皮肤的角质形成细胞。此外，白癜风表皮基底膜中的局灶性间隙被解释为黑素细胞外改变的证据。为了了解这些间隙的发生情况，我们开展了一项研究，以确定哪些细胞与它们有关。采用免疫荧光和免疫过氧化物酶技术，在受累皮肤、未受累皮肤和对照皮肤中观察基底膜、黑素细胞和默克尔细胞。用荧光素偶联的抗IV型胶原抗体和罗丹明偶联的针对半桥粒成分尼斯因（GB3）的抗体进行双重染色。用碘化丙啶染色检测浸润淋巴细胞。结果发现基底膜结构始终完整且未改变。未受累皮肤区域无染色与默克尔细胞、黑素细胞和浸润淋巴细胞的存在有关。因此，表皮基底膜中的间隙是由于迁移细胞的存在导致染色缺失所致。
