Atzpodien E, Baumgärtner W, Artelt A, Thiele D
Institut für Veterinär-Pathologie, Justus-Liebig-Universität Giessen, Germany.
J Infect Dis. 1994 Jul;170(1):223-6. doi: 10.1093/infdis/170.1.223.
BALB/cJ (H-2d) mice were injected intraperitoneally (ip) with cyclophosphamide 2 days after ip inoculation with Coxiella burnetii Nine Mile, phase I. Ten days after infection, disseminated microabscesses, granulomas, and microthrombi were observed in most organs, including spleen and liver, and in bone marrow. In addition, endocarditis of the atrioventricular and semilunar valves, characterized by macrophages and neutrophils, was present. At the same time, C. burnetii antigen was found in most organs and in cardiac valves, aorta, and pulmonary artery, mainly within macrophages, neutrophils, and endothelial cells. C. burnetii-specific IgG antibodies were detected 150 days after infection but not in the early phase of infection. Cardiac valves were devoid of C. burnetii antigen and without residual lesions at 150 days. These findings offer the possibility for further studies on the pathogenesis of C. burnetii-associated valvular endocarditis and show that acute strains of C. burnetii can cause endocarditis as a part of a disseminated infection in the immunocompromised host.
在腹腔接种第一阶段的伯纳特柯克斯体Nine Mile株2天后，对BALB/cJ (H-2d)小鼠进行腹腔注射环磷酰胺。感染10天后，在包括脾脏、肝脏和骨髓在内的大多数器官中观察到弥漫性微脓肿、肉芽肿和微血栓。此外，存在以巨噬细胞和中性粒细胞为特征的房室瓣和半月瓣心内膜炎。同时，在大多数器官以及心脏瓣膜、主动脉和肺动脉中发现了伯纳特柯克斯体抗原，主要存在于巨噬细胞、中性粒细胞和内皮细胞内。感染150天后检测到伯纳特柯克斯体特异性IgG抗体，但在感染早期未检测到。150天时心脏瓣膜没有伯纳特柯克斯体抗原且无残留病变。这些发现为进一步研究伯纳特柯克斯体相关瓣膜性心内膜炎的发病机制提供了可能性，并表明伯纳特柯克斯体急性菌株可作为免疫受损宿主播散性感染的一部分引起心内膜炎。
