一种作为短散在重复元件（SINEs）起源的新型tRNA种类。马的SINEs可能起源于tRNA(Ser)。

A novel tRNA species as an origin of short interspersed repetitive elements (SINEs). Equine SINEs may have originated from tRNA(Ser).

作者信息

Sakagami M, Ohshima K, Mukoyama H, Yasue H, Okada N

机构信息

Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association, Tokyo.

出版信息

J Mol Biol. 1994 Jun 24;239(5):731-5. doi: 10.1006/jmbi.1994.1410.

DOI:10.1006/jmbi.1994.1410

PMID:8014993

Abstract

Short interspersed repetitive elements (SINEs) were isolated from the equine genome and characterized. The equine SINE (ERE-1) family has several features characteristic of tRNA-derived retroposons. The five members of the equine family of SINEs are approximately 230 nucleotides in length and terminate with a sequence rich in oligo(A). They are all flanked by direct repeats at the 5' and 3' ends, and such repeats are the hallmarks of retroposons. In addition, the ERE-1 family has a tRNA-related region, which is similar to tRNA(Ser) of Drosophila (65% identity). tRNA(Ser) is a novel tRNA with respect to the origin of SINEs and has not previously been recognized among the twenty tRNA-derived SINEs characterized to date. The members of the ERE-1 family were found to be distributed among five species in the genus Equus, and their amplification may have contributed to the genetic variability of their hosts during evolution.

摘要

短散在重复元件（SINEs）从马基因组中分离出来并进行了特征分析。马SINE（ERE-1）家族具有一些源自tRNA的反转录转座子的特征。马SINE家族的五个成员长度约为230个核苷酸，并以富含寡聚腺苷酸的序列结尾。它们在5'和3'末端均侧翼有正向重复序列，而这种重复序列是反转录转座子的标志。此外，ERE-1家族有一个与tRNA相关的区域，与果蝇的tRNA(Ser)相似（同一性为65%）。就SINEs的起源而言，tRNA(Ser)是一种新型tRNA，在迄今已鉴定的20种源自tRNA的SINEs中尚未被识别。发现ERE-1家族的成员分布在马属的五个物种中，它们的扩增可能在进化过程中对其宿主的遗传变异性有所贡献。