果蝠中源自5S rRNA和tRNA的短散在重复序列

5S rRNA-derived and tRNA-derived SINEs in fruit bats.

作者信息

Gogolevsky Konstantin P, Vassetzky Nikita S, Kramerov Dmitri A

机构信息

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., Moscow 119991, Russia.

出版信息

Genomics. 2009 May;93(5):494-500. doi: 10.1016/j.ygeno.2009.02.001. Epub 2009 Feb 12.

DOI:10.1016/j.ygeno.2009.02.001

PMID:19442632

Abstract

Most short retroposons (SINEs) descend from cellular tRNA of 7SL RNA. Here, four new SINEs were found in megabats (Megachiroptera) but neither in microbats nor in other mammals. Two of them, MEG-RS and MEG-RL, descend from another cellular RNA, 5S rRNA; one (MEG-T2) is a tRNA-derived SINE; and MEG-TR is a hybrid tRNA/5S rRNA SINE. Insertion locus analysis suggests that these SINEs were active in the recent fruit bat evolution. Analysis of MEG-RS and MEG-RL in comparison with other few 5S rRNA-derived SINEs demonstrates that the internal RNA polymerase III promoter is their most invariant region, while the secondary structure is more variable. The mechanisms underlying the modular structure of these and other SINEs as well as their variation are discussed. The scenario of evolution of MEG SINEs is proposed.

摘要

大多数短散在重复元件（SINEs）起源于7SL RNA的细胞tRNA。在此，在大蝙蝠（Megachiroptera）中发现了四种新的SINEs，但在小蝙蝠和其他哺乳动物中均未发现。其中两种，MEG-RS和MEG-RL，起源于另一种细胞RNA，即5S rRNA；一种（MEG-T2）是tRNA衍生的SINE；而MEG-TR是一种tRNA/5S rRNA杂合SINE。插入位点分析表明，这些SINEs在最近的果蝠进化过程中是活跃的。与其他少数5S rRNA衍生的SINEs相比，对MEG-RS和MEG-RL的分析表明，内部RNA聚合酶III启动子是它们最保守的区域，而二级结构则更具变异性。讨论了这些SINEs和其他SINEs模块化结构及其变异的潜在机制。提出了MEG SINEs的进化模式。