Vidovic M, Cohen D, Hill C E
Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Brain Res Mol Brain Res. 1994 Mar;22(1-4):49-56. doi: 10.1016/0169-328x(94)90031-0.
alpha 2 Adrenergic receptors are involved in mediating pre- and postsynaptic responses in the sympathetic nervous system. In this study, the expression of alpha 2 genes was examined by the amplification of mRNA, extracted from adult rat superior cervical ganglion through reverse transcription and subsequent amplification of appropriate target sequence using polymerase chain reaction and sequence specific oligonucleotide primers for the three alpha 2 receptor genes. Results from these studies have shown that the major alpha 2 adrenergic mRNA transcript was the one that encodes the alpha 2A receptor. Nucleotide sequence of the 312 base-pair (bp) alpha 2A cDNA was homologous to the RG20 adrenergic receptor, the rat homologue of the human alpha 2A receptor. The 312 bp alpha 2A cDNA was used as a probe in Northern blot analysis of the mRNA from superior cervical ganglion and brain. A 3.9 kb mRNA transcript was present in these extracts. To confirm that the alpha 2A gene expression was in the sympathetic neurones we have used the 312 bp alpha 2A cDNA, biotinylated, as a probe for in situ hybridization studies and have demonstrated that the alpha 2A mRNA was found only in the cell bodies of sympathetic neurones.
α2肾上腺素能受体参与介导交感神经系统突触前和突触后的反应。在本研究中，通过从成年大鼠颈上神经节提取mRNA进行扩增来检测α2基因的表达，该mRNA通过逆转录获得，随后使用聚合酶链反应和针对三个α2受体基因的序列特异性寡核苷酸引物扩增适当的靶序列。这些研究结果表明，主要的α2肾上腺素能mRNA转录本是编码α2A受体的转录本。312个碱基对（bp）的α2A cDNA的核苷酸序列与RG20肾上腺素能受体同源，RG20是人类α2A受体的大鼠同源物。312 bp的α2A cDNA用作颈上神经节和大脑mRNA的Northern印迹分析中的探针。这些提取物中存在一个3.9 kb的mRNA转录本。为了证实α2A基因在交感神经元中表达，我们使用生物素化的312 bpα2A cDNA作为原位杂交研究的探针，并证明α2A mRNA仅在交感神经元的细胞体中发现。
