Radioimmunoassay of the gastrointestinal hormones.

The principle and types of saturation analysis are discussed on the basis of data in the literature. This is followed by summarizing the main steps of radioimmunoassay (RIA) of gastrointestinal hormones. The normal fasting blood levels of these hormones are also given. RIA measurement of the individual hormones is discussed critically, and attention is called to the theoretical importance of immune cross-reactions. The RIA model developed by the author for measuring the C-terminal pentapeptide of gastrin is demonstrated.