Differences in biochemical profile from active and inactive ovarian tissue in relation to oestrous cycle phases.

The differences in biochemical components between active and inactive ovarian tissue were studied during the oestrous cycle. Goats (Marwari breed) were slaughtered at six different phases of the oestrous cycle (n = 5) such as oestrus (day 1), ovulatory (day 2), early luteal (day 3), late luteal (day 9), follicular (day 15) phase, and goats having smooth ovaries without follicles or CL (SO). The levels of total protein, soluble protein, alkaline and acid phosphatase were significantly different (P < 0.05) at different phases of oestrous cycle. Differences in nucleic acids (RNA and DNA), ascorbic acid, free cholesterol and esterified cholesterol were non-significant. The levels of proteins (total and soluble) and nucleic acids were high in the active ovary on day 9 (peak luteal phase) and day 15 (follicular phase) of the cycle. The activity of both alkaline and acid phosphatases was higher on day 2 (ovulatory phase) in the active ovary as compared with the inactive ovary. Highly significant (P < 0.01) differences in total cholesterol concentration were found between different phases of the oestrous cycle. Maximum utilization of cholesterol was observed in the peak luteal phase (day 9) of the cycle.