Sureshkumar P K, Janakiraman K
Reproductive Biology Research Unit, Gujrat Agriculture University, Anand, India.
Acta Vet Hung. 1993;41(3-4):301-13.
The differences in biochemical components between active and inactive ovarian tissue were studied during the oestrous cycle. Goats (Marwari breed) were slaughtered at six different phases of the oestrous cycle (n = 5) such as oestrus (day 1), ovulatory (day 2), early luteal (day 3), late luteal (day 9), follicular (day 15) phase, and goats having smooth ovaries without follicles or CL (SO). The levels of total protein, soluble protein, alkaline and acid phosphatase were significantly different (P < 0.05) at different phases of oestrous cycle. Differences in nucleic acids (RNA and DNA), ascorbic acid, free cholesterol and esterified cholesterol were non-significant. The levels of proteins (total and soluble) and nucleic acids were high in the active ovary on day 9 (peak luteal phase) and day 15 (follicular phase) of the cycle. The activity of both alkaline and acid phosphatases was higher on day 2 (ovulatory phase) in the active ovary as compared with the inactive ovary. Highly significant (P < 0.01) differences in total cholesterol concentration were found between different phases of the oestrous cycle. Maximum utilization of cholesterol was observed in the peak luteal phase (day 9) of the cycle.
在发情周期中，研究了活跃和不活跃卵巢组织之间生化成分的差异。选用了山羊（马尔瓦里品种），在发情周期的六个不同阶段（n = 5）进行屠宰，这些阶段包括发情期（第1天）、排卵期（第2天）、黄体早期（第3天）、黄体晚期（第9天）、卵泡期（第15天），以及卵巢表面光滑无卵泡或黄体的山羊（SO）。在发情周期的不同阶段，总蛋白、可溶性蛋白、碱性磷酸酶和酸性磷酸酶的水平存在显著差异（P < 0.05）。核酸（RNA和DNA）、抗坏血酸、游离胆固醇和酯化胆固醇的差异不显著。在周期的第9天（黄体高峰期）和第15天（卵泡期），活跃卵巢中的蛋白质（总蛋白和可溶性蛋白）和核酸水平较高。与不活跃卵巢相比，活跃卵巢在第2天（排卵期）时碱性磷酸酶和酸性磷酸酶的活性均较高。在发情周期的不同阶段，总胆固醇浓度存在极显著差异（P < 0.01）。在周期的黄体高峰期（第9天）观察到胆固醇的最大利用率。
