Young D W, Kemppainen R J
Department of Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, AL 36849-5520.
Am J Vet Res. 1994 Apr;55(4):567-71.
Acid extracts of anterior and intermediate lobes of the pituitary gland from 4 dogs were fractionated by cation-exchange high-performance liquid chromatography and analyzed by radioimmunoassay, using 2 antibodies: one specific for the midregion of beta-endorphin (beta-end) and the other specific for the N-terminal region of N-acetylated beta-END. Identification of peaks of canine beta-END immunoreactivity was based on the retention times relative to those of synthetic human beta-END standards, with predicted variations attributable to differences in the beta-END amino acid sequences between the 2 species. The canine anterior lobe was found to contain almost exclusively beta-END (1-31). By contrast, the intermediate lobe contained substantial amounts of N-acetylated and C-terminally shortened forms of beta-END. The predominant forms of beta-END in canine intermediate lobe were, in decreasing order of abundance: beta-END (1-27), beta-END (1-31), beta-END (1-26), Ac beta-END (1-27), Ac beta-END (1-26) and Ac beta-END (1-31). Individual beta-END immunoreactivity profiles varied, but the general pattern was consistent among the 4 dogs.
对4只犬垂体前叶和中叶的酸性提取物进行阳离子交换高效液相色谱分级分离，并用两种抗体通过放射免疫分析进行检测：一种对β-内啡肽（β-end）的中间区域具有特异性，另一种对N-乙酰化β-END的N端区域具有特异性。犬β-END免疫反应性峰的鉴定基于相对于合成人β-END标准品的保留时间，预测的差异归因于这两个物种之间β-END氨基酸序列的不同。发现犬垂体前叶几乎只含有β-END（1-31）。相比之下，中叶含有大量N-乙酰化和C端缩短形式的β-END。犬中叶中β-END的主要形式按丰度递减顺序为：β-END（1-27）、β-END（1-31）、β-END（1-26）、Acβ-END（1-27）、Acβ-END（1-26）和Acβ-END（1-31）。个体β-END免疫反应性谱有所不同，但4只犬之间的总体模式是一致的。
