Stewart P S, Griebe T, Srinivasan R, Chen C I, Yu F P, deBeer D, McFeters G A
Center for Biofilm Engineering, Montana State University, Bozeman 59717.
Appl Environ Microbiol. 1994 May;60(5):1690-2. doi: 10.1128/aem.60.5.1690-1692.1994.
Biofilm bacteria challenged with monochloramine retained significant respiratory activity, even though they could not be cultured on agar plates. Microbial colony counts on agar media declined by approximately 99.9% after 1 h of disinfection, whereas the number of bacteria stained by a fluorescent redox dye experienced a 93% reduction. Integrated measures of biofilm respiratory activity, including net oxygen and glucose utilization rates, showed only a 10 to 15% reduction. In this biofilm system, measures of microbial respiratory activity and culturability yielded widely differing estimates of biocide efficacy.
尽管受到一氯胺攻击的生物膜细菌无法在琼脂平板上培养，但它们仍保留了显著的呼吸活性。消毒1小时后，琼脂培养基上的微生物菌落数下降了约99.9%，而用荧光氧化还原染料染色的细菌数量减少了93%。包括净氧利用率和葡萄糖利用率在内的生物膜呼吸活性综合指标仅下降了10%至15%。在这个生物膜系统中，微生物呼吸活性和可培养性的测量结果对杀菌剂效果的估计差异很大。
