Wallach F R, Forni A L, Hariprashad J, Stoeckle M Y, Steinberg C R, Fisher L, Malawista S E, Murray H W
Lyme Disease Research Laboratory, Cornell University Medical College, New York, New York.
J Infect Dis. 1993 Dec;168(6):1541-3. doi: 10.1093/infdis/168.6.1541.
To determine the usefulness of blood culture and polymerase chain reaction (PCR) analysis in detecting circulating Borrelia burgdorferi or its DNA, blood and serum from untreated patients with acute Lyme disease were examined. None of the cultures of blood or serum from the 7 patients tested demonstrated spirochetes. Similarly, all patient serum samples, assayed in two laboratories, were negative for B. burgdorferi DNA using PCR amplification. These results suggest that in patients with acute Lyme disease, spirochetes, spirochete DNA, or both circulate early, only intermittently, or at low levels and that neither culture nor PCR testing of blood or serum, as currently done, appears likely to prove generally useful in the diagnosis of Lyme disease.
为了确定血培养和聚合酶链反应（PCR）分析在检测循环中的伯氏疏螺旋体或其DNA方面的效用，对未经治疗的急性莱姆病患者的血液和血清进行了检测。检测的7例患者的血液或血清培养物均未发现螺旋体。同样，在两个实验室检测的所有患者血清样本，通过PCR扩增检测伯氏疏螺旋体DNA均为阴性。这些结果表明，在急性莱姆病患者中，螺旋体、螺旋体DNA或两者在疾病早期循环，只是间歇性地或处于低水平，并且目前所进行的血液或血清培养及PCR检测，似乎都不太可能普遍用于莱姆病的诊断。
