Heidrich P, Wink M
Universität Heidelberg, Institut für Pharmazeutische Biologie.
Z Naturforsch C J Biosci. 1994 Mar-Apr;49(3-4):230-4. doi: 10.1515/znc-1994-3-411.
The cytochrome b gene of the Tawny Owl (Strix aluco), Hume's Tawny Owl (Strix butleri) and the African wood owl (Strix woodfordii) was amplified by polymerase chain reaction (PCR) and partially sequenced (300 base pairs). Sequences differ substantially (9 to 12% nucleotide substitutions) between these taxa indicating that they represent distinct species, which is also implicated from morphological and biogeographic differences. Using cytochrome b sequences of S. aluco, S. butleri, S. woodfordii, Athene noctua and Tyto alba phylogenetic relationship were reconstructed using the "maximum parsimony" principal (PAUP 3.1.1) and the neighbour-joining method (MEGA).
通过聚合酶链反应（PCR）扩增了灰林鸮（Strix aluco）、褐林鸮（Strix butleri）和非洲林鸮（Strix woodfordii）的细胞色素b基因，并进行了部分测序（300个碱基对）。这些分类单元之间的序列差异很大（9%至12%的核苷酸替换），表明它们代表不同的物种，这也从形态学和生物地理学差异中得到暗示。利用灰林鸮、褐林鸮、非洲林鸮、纵纹腹小鸮（Athene noctua）和草鸮（Tyto alba）的细胞色素b序列，采用“最大简约法”原理（PAUP 3.1.1）和邻接法（MEGA）重建了系统发育关系。
