Sonenberg N
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Gene Expr. 1993;3(3):317-23.
It is evident from the data discussed here that the mechanism and the rules for mRNA binding in eukaryotes are complex and not well defined. The major points of this review are (1) ribosome binding could be preceded by the unwinding of mRNA secondary structure; (2) there is no obligatory ribosome entry through the 5' end of the mRNA; (3) there is no obligatory linear "scanning" of the 5'UTR; and (4) there are some interesting similarities between prokaryotes and eukaryotes in the mode of ribosome binding to mRNA, particularly in the ability of the small ribosomal subunit to diffuse or "scan" on the mRNA, and in the requirement for a minimally structured RNA for efficient ribosome binding.
从这里讨论的数据可以明显看出，真核生物中mRNA结合的机制和规则很复杂且尚未明确界定。本综述的要点如下：（1）核糖体结合可能先于mRNA二级结构的解旋；（2）核糖体并非必须通过mRNA的5'端进入；（3）不存在对5'UTR进行强制性线性“扫描”的情况；（4）原核生物和真核生物在核糖体与mRNA结合模式上存在一些有趣的相似之处，特别是小核糖体亚基在mRNA上扩散或“扫描”的能力，以及高效核糖体结合对最小结构化RNA的要求。
