Fairbairn D W, Rowe K C, O'Neill K L
Department of Microbiology, Brigham Young University, Provo, Utah 84602.
Cell Biol Int. 1994 Mar;18(3):195-200. doi: 10.1006/cbir.1994.1061.
Laser Scanning Microscopy is a sensitive tool that provides a unique method of analyzing biological systems. Coupled with the Single Cell Gel assay, it allows for accurate and reproducible detection of DNA strand breaks. An understanding of the theory of DNA comet formation is lacking. Using dexamethasone induced apoptosis in murine thymocytes as a model for double strand breaks, we used video enhanced laser scanning microscopy to evaluate the leading edge of DNA migration in the single cell gel assay. In this system, comet length increases significantly within the first thirty seconds of electrophoresis, the greatest increase in length is completed within the first minute, and the first two minutes are important in significant increases in DNA migration during DNA comet formation.
激光扫描显微镜是一种灵敏的工具，它提供了一种分析生物系统的独特方法。与单细胞凝胶试验相结合，它能够准确且可重复地检测DNA链断裂。目前尚缺乏对DNA彗星形成理论的理解。我们以地塞米松诱导小鼠胸腺细胞凋亡作为双链断裂的模型，使用视频增强激光扫描显微镜来评估单细胞凝胶试验中DNA迁移的前沿。在这个系统中，彗星长度在电泳的前30秒内显著增加，长度的最大增加在第一分钟内完成，并且前两分钟对于DNA彗星形成过程中DNA迁移的显著增加很重要。
