Wang Y Y, Cutz E, Perrin D G
Department of Pathology, Hospital for Sick Children, Toronto, Ontario, Canada.
Cell Tissue Res. 1994 May;276(2):399-402. doi: 10.1007/BF00306125.
Calcitonin mRNA was detected in human and monkey carotid bodies by in situ hybridization histochemistry, using a 35S-labeled oligonucleotide probe for human calcitonin. In both human and monkey carotid body, moderate to high hybridization signal for calcitonin mRNA was observed in all cases. The hybridization signal in the formalin-fixed, paraffin-embedded samples was comparable to that obtained from frozen paraformaldehyde-fixed tissue. Our observations extend the finding of calcitonin-like immunoreactivity in the carotid body chief cells and indicate that calcitonin is produced in the carotid body, probably in the chief cells.
采用针对人降钙素的35S标记寡核苷酸探针，通过原位杂交组织化学技术在人和猴的颈动脉体中检测到降钙素mRNA。在人和猴的颈动脉体中，所有病例均观察到降钙素mRNA的中度至高杂交信号。福尔马林固定、石蜡包埋样本中的杂交信号与从冷冻多聚甲醛固定组织中获得的信号相当。我们的观察结果扩展了颈动脉体主细胞中降钙素样免疫反应性的发现，并表明降钙素在颈动脉体中产生，可能在主细胞中。
