Thomas B J, Gunning D A, Cho J, Zipursky L
Department of Biological Chemistry, University of California, Los Angeles 90024.
Cell. 1994 Jul 1;77(7):1003-14. doi: 10.1016/0092-8674(94)90440-5.
The onset of pattern formation in the developing Drosophila eye is marked by the simultaneous synchronization of all cells in the G1 phase of the cell cycle. These cells will then either commit to another round of cell division or differentiate into neurons. Although cell cycle synchronization occurs in roughex (rux) mutants, cells circumvent G1 and all cells enter S phase, including cells that would normally differentiate. This leads to defects in early steps of pattern formation and cell fate determination. rux is suppressed by mutations in genes that promote cell cycle progression (i.e., cyclin A and string) and enhanced by mutations in genes that promote differentiation (i.e., Ras1 and Star). rux encodes a novel protein of 335 amino acids. We propose that rux functions as a negative regulator of G1 progression in the developing eye.
果蝇发育中的眼睛图案形成的起始以细胞周期G1期所有细胞的同时同步化为标志。这些细胞随后要么进入新一轮细胞分裂，要么分化为神经元。尽管在粗糙眼（rux）突变体中发生了细胞周期同步化，但细胞绕过G1期，所有细胞包括通常会分化的细胞都进入S期。这导致图案形成和细胞命运决定早期步骤出现缺陷。促进细胞周期进程的基因（即细胞周期蛋白A和String）的突变会抑制rux，而促进分化的基因（即Ras1和Star）的突变会增强rux。rux编码一种由335个氨基酸组成的新型蛋白质。我们提出rux在发育中的眼睛中作为G1期进程的负调节因子发挥作用。
