• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

The yeast KEM1 gene encodes a nuclease specific for G4 tetraplex DNA: implication of in vivo functions for this novel DNA structure.

作者信息

Liu Z, Gilbert W

机构信息

Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.

出版信息

Cell. 1994 Jul 1;77(7):1083-92. doi: 10.1016/0092-8674(94)90447-2.

DOI:10.1016/0092-8674(94)90447-2
PMID:8020096
Abstract

We have previously reported the identification of a G4-DNA-dependent nuclease from S. cerevisiae that recognizes a tetrastranded G4-DNA structure and cuts in a single-stranded region 5' to the G4 structure. We purify this activity to homogeneity and show it to be the product of the S. cerevisiae KEM1 gene, which is also known as SEP1, DST2, XRN1, and RAR5. Since a homozygous deletion of the KEM1 gene blocks meiotic cells at the 4N stage, the finding of these G4-dependent DNA binding and cleavage activities for the KEM1 gene product supports the hypothesis that G4-DNA may play a role in meiosis.

摘要

相似文献

1
The yeast KEM1 gene encodes a nuclease specific for G4 tetraplex DNA: implication of in vivo functions for this novel DNA structure.
Cell. 1994 Jul 1;77(7):1083-92. doi: 10.1016/0092-8674(94)90447-2.
2
Gene disruption of a G4-DNA-dependent nuclease in yeast leads to cellular senescence and telomere shortening.
Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6002-6. doi: 10.1073/pnas.92.13.6002.
3
Identification of functional domains in the Sep1 protein (= Kem1, Xrn1), which is required for transition through meiotic prophase in Saccharomyces cerevisiae.鉴定酿酒酵母减数分裂前期转换所必需的Sep1蛋白（=Kem1，Xrn1）中的功能结构域。
Chromosoma. 1995 Nov;104(3):215-22. doi: 10.1007/BF00352186.
4
ROK1, a high-copy-number plasmid suppressor of kem1, encodes a putative ATP-dependent RNA helicase in Saccharomyces cerevisiae.ROK1是kem1的一种高拷贝数质粒抑制因子，在酿酒酵母中编码一种假定的ATP依赖性RNA解旋酶。
Gene. 1995 Dec 1;166(1):151-4. doi: 10.1016/0378-1119(96)80010-2.
5
Structure of the yeast TAP1 protein: dependence of transcription activation on the DNA context of the target gene.酵母TAP1蛋白的结构：转录激活对靶基因DNA环境的依赖性。
Mol Cell Biol. 1993 Jun;13(6):3434-44. doi: 10.1128/mcb.13.6.3434-3444.1993.
6
Regulation and intracellular localization of Saccharomyces cerevisiae strand exchange protein 1 (Sep1/Xrn1/Kem1), a multifunctional exonuclease.酿酒酵母链交换蛋白1（Sep1/Xrn1/Kem1）的调控及细胞内定位，一种多功能核酸外切酶
Mol Cell Biol. 1995 May;15(5):2728-36. doi: 10.1128/MCB.15.5.2728.
7
Requirement of S. pombe exonuclease II, a homologue of S. cerevisiae Sep1, for normal mitotic growth and viability.
Curr Genet. 1996 Sep;30(4):284-93. doi: 10.1007/s002940050134.
8
The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes.酿酒酵母七号染色体左臂上一个11.1 kb片段的序列揭示了六个开放阅读框，包括NSP49、KEM1和四个假定的新基因。
Yeast. 1995 Sep 30;11(12):1187-94. doi: 10.1002/yea.320111209.
9
Use of monoclonal antibodies in the functional characterization of the Saccharomyces cerevisiae Sep1 protein.
Eur J Biochem. 1995 Jul 15;231(2):329-36. doi: 10.1111/j.1432-1033.1995.tb20704.x.
10
Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA.RAT1的分离与鉴定：酿酒酵母中一个对mRNA高效核质运输所必需的关键基因
Genes Dev. 1992 Jul;6(7):1173-89. doi: 10.1101/gad.6.7.1173.

引用本文的文献

1
Impact of G-Quadruplexes on the Regulation of Genome Integrity, DNA Damage and Repair.G-四链体对基因组完整性、DNA 损伤与修复调控的影响
Biomolecules. 2021 Aug 27;11(9):1284. doi: 10.3390/biom11091284.
2
G-Quadruplex Structures Colocalize with Transcription Factories and Nuclear Speckles Surrounded by Acetylated and Dimethylated Histones H3.G-四链体结构与转录因子和被乙酰化和二甲基化组蛋白 H3 包围的核斑点共定位。
Int J Mol Sci. 2021 Feb 17;22(4):1995. doi: 10.3390/ijms22041995.
3
G-quadruplexes may determine the landscape of recombination in HSV-1.
G-四链体可能决定 HSV-1 中的重组景观。
BMC Genomics. 2019 May 16;20(1):382. doi: 10.1186/s12864-019-5731-0.
4
Mms1 is an assistant for regulating G-quadruplex DNA structures.Mms1是一种调节G-四链体DNA结构的辅助因子。
Curr Genet. 2018 Jun;64(3):535-540. doi: 10.1007/s00294-017-0773-9. Epub 2017 Nov 2.
5
Red1 protein exhibits nonhomologous DNA end-joining activity and potentiates Hop1-promoted pairing of double-stranded DNA.Red1蛋白具有非同源DNA末端连接活性，并增强Hop1促进的双链DNA配对。
J Biol Chem. 2017 Aug 18;292(33):13853-13866. doi: 10.1074/jbc.M117.796425. Epub 2017 Jun 22.
6
Comprehensive classification of the PIN domain-like superfamily.PIN结构域样超家族的综合分类
Nucleic Acids Res. 2017 Jul 7;45(12):6995-7020. doi: 10.1093/nar/gkx494.
7
Probing the Potential Role of Non-B DNA Structures at Yeast Meiosis-Specific DNA Double-Strand Breaks.探究非B型DNA结构在酵母减数分裂特异性DNA双链断裂中的潜在作用。
Biophys J. 2017 May 23;112(10):2056-2074. doi: 10.1016/j.bpj.2017.04.028.
8
G-quadruplexes and their regulatory roles in biology.G-四链体及其在生物学中的调控作用。
Nucleic Acids Res. 2015 Oct 15;43(18):8627-37. doi: 10.1093/nar/gkv862. Epub 2015 Sep 8.
9
G-quadruplexes in viruses: function and potential therapeutic applications.病毒中的G-四链体：功能及潜在治疗应用
Nucleic Acids Res. 2014 Nov 10;42(20):12352-66. doi: 10.1093/nar/gku999. Epub 2014 Oct 20.
10
Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity.哺乳动物 DNA2 解旋酶/核酸酶可切割 G-四链体 DNA，并且对于端粒的完整性是必需的。
EMBO J. 2013 May 15;32(10):1425-39. doi: 10.1038/emboj.2013.88. Epub 2013 Apr 19.