Gaetje R
Department of Obstetrics and Gynecology, Johann Wolfgang Goethe, University Clinic, Frankfurt, Germany.
Clin Exp Obstet Gynecol. 1994;21(1):14-23.
The effect of IGF-I, EGF, PDGF and FGF on human granulosa cells of IVF-cycles, and the effect of IGF-I on granulosa cells of natural cycles (day 7 to 13 of cycle) were evaluated in vitro with and without hCG stimulation. At concentrations of 1 ng and 5 ng per ml culture FGF and PDGF did not alter progesterone and estradiol secretion or the morphology of preovulatory granulosa cells. At 1 ng, 2 ng, and 3 ng per ml medium EGF significantly enhanced basal progesterone and estradiol secretion and significantly decreased hCG stimulated estradiol production of preovulatory granulosa cells. While IGF-I treatment with and without hCG stimulation did not alter steroid secretion of preovulatory granulosa cells, the progesterone secretion of granulosa cells of natural cycles was increased by combined treatment with 10 I.U. hCG + 25 ng IGF-I per ml culture medium. Increased steroid secretion was related to reduced cell spreading. Our results provide evidence for the facultative role of IGF-I and EGF as an autocrine/paracrine modulator of ovarian function. IGF-I may play a role in regulation of ovulation induction and luteinisation as IGF-I and hCG act synergistically in increasing progesterone secretion of granulosa cells of natural cycles in vitro and are known to stimulate each other's receptor expression.
在有无hCG刺激的情况下，体外评估了IGF-I、EGF、PDGF和FGF对体外受精周期人颗粒细胞的影响，以及IGF-I对自然周期（周期第7至13天）颗粒细胞的影响。在每毫升培养基1 ng和5 ng的浓度下，FGF和PDGF不会改变孕酮和雌二醇的分泌，也不会改变排卵前颗粒细胞的形态。在每毫升培养基1 ng、2 ng和3 ng的浓度下，EGF显著增强基础孕酮和雌二醇的分泌，并显著降低hCG刺激的排卵前颗粒细胞的雌二醇产生。虽然有无hCG刺激时IGF-I处理均未改变排卵前颗粒细胞的类固醇分泌，但每毫升培养基联合使用10 I.U. hCG + 25 ng IGF-I处理可增加自然周期颗粒细胞的孕酮分泌。类固醇分泌增加与细胞铺展减少有关。我们的结果为IGF-I和EGF作为卵巢功能自分泌/旁分泌调节剂的兼性作用提供了证据。IGF-I可能在排卵诱导和黄体化调节中发挥作用，因为IGF-I和hCG在体外协同增加自然周期颗粒细胞的孕酮分泌，并且已知它们会刺激彼此受体的表达。
