Ganeva R, Spassovska N, Genchev D
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Cytobios. 1994;77(309):81-6.
The in vitro and in vivo effects of cis-diamminedichloroplatinum(II) (cis-DDP), transdiamminedichloroplatinum(II), spermine-platinum(II) complex and K2PtCl4 on the RNA polymerase reaction in isolated nuclei from Ehrlich ascites tumour cells were investigated. Incubation of nuclei in the presence of platinum compounds did not substantially influence the RNA polymerase reaction. The results from the in vivo experiments (i.e. after i.p. injection of mice with platinum compounds before nuclei isolation) showed that the compounds displayed distinct inhibitory effects on the reaction which was most pronounced with K2PtCl4 and cis-DDP.
研究了顺二氯二氨合铂（II）（顺铂）、反二氯二氨合铂（II）、精胺-铂（II）配合物和四氯合铂酸钾（K2PtCl4）对艾氏腹水瘤细胞分离细胞核中RNA聚合酶反应的体外和体内效应。在铂化合物存在下孵育细胞核对RNA聚合酶反应没有实质性影响。体内实验结果（即在分离细胞核前经腹腔注射铂化合物给小鼠）表明，这些化合物对该反应具有明显的抑制作用，其中K2PtCl4和顺铂的抑制作用最为显著。
