荚膜红细菌rpmF - plsX - fabH基因的鉴定。

Identification of the rpmF-plsX-fabH genes of Rhodobacter capsulatus.

作者信息

Carty S M, Colbeau A, Vignais P M, Larson T J

机构信息

Department of Biochemistry and Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061-0308.

出版信息

FEMS Microbiol Lett. 1994 May 15;118(3):227-31. doi: 10.1111/j.1574-6968.1994.tb06832.x.

DOI:10.1111/j.1574-6968.1994.tb06832.x

PMID:8020746

Abstract

The rpmF-plsX-fabH gene cluster of Rhodobacter capsulatus homologous to that of Escherichia coli was identified. rpmF encodes ribosomal protein L32, plsX plays an undefined role in membrane lipid synthesis, and fabH encodes beta-ketoacyl-acyl carrier protein synthase III. The R. capsulatus plsX gene complemented a defect in an E. coli strain with the plsX50 mutation. Overproduction of the fabH gene product of R. capsulatus in E. coli resulted in dramatically increased beta-ketoacyl-acyl carrier protein synthase III activity. These results indicate that plsX and fabH apparently function the same in R. capsulatus as in E. coli.

摘要

已鉴定出与大肠杆菌同源的荚膜红细菌的rpmF-plsX-fabH基因簇。rpmF编码核糖体蛋白L32，plsX在膜脂合成中发挥未明确的作用，fabH编码β-酮酰基-酰基载体蛋白合酶III。荚膜红细菌的plsX基因弥补了大肠杆菌plsX50突变菌株的缺陷。在大肠杆菌中过量表达荚膜红细菌的fabH基因产物导致β-酮酰基-酰基载体蛋白合酶III活性显著增加。这些结果表明，plsX和fabH在荚膜红细菌中的功能显然与在大肠杆菌中相同。