Lemez P, Jelínek J, Michalová K, Koubek K, Schwarz J, Malasková V, Rypácková B, Jirásek A, Brezinová J, Hrabánek J
Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
Leuk Res. 1994 Jul;18(7):493-7. doi: 10.1016/0145-2126(94)90087-6.
Leukemic blasts of two patients with acute leukemia exhibited similar characteristics. They were heterogeneous in size with a diameter of 14-30 microns in smears and unclassifiable by morphological, cytochemical, immunophenotypic and ultrastructural examinations. Cytogenetic examinations of both revealed a near-tetraploid karyotype. Blasts from both patients differentiated into macrophages in cultures with 10 ng/ml 12-O-tetradecanoylphorbol-13-acetate (TPA) which is a feature specific for myeloid blasts and the cases were thus classified as poorly differentiated acute myeloid leukemias (AML M0). Near-tetraploid poorly differentiated acute myeloid leukemias M0 seem to be a special category of AML in the morphologic, immunologic and cytogenetic (MIC) classification. The presence of very large blasts in the heterogeneous blast population in acute unclassified leukemias could be a morphological sign of near-tetraploid leukemias AML M0.
两名急性白血病患者的白血病原始细胞表现出相似特征。它们大小不均一，涂片直径为14 - 30微米，通过形态学、细胞化学、免疫表型和超微结构检查无法分类。两者的细胞遗传学检查均显示近四倍体核型。两名患者的原始细胞在含有10 ng/ml 12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）的培养物中分化为巨噬细胞，这是髓系原始细胞的一个特征性表现，因此这些病例被归类为低分化急性髓系白血病（AML M0）。近四倍体低分化急性髓系白血病M0在形态学、免疫学和细胞遗传学（MIC）分类中似乎是AML的一个特殊类别。急性未分类白血病中异质性原始细胞群体中存在非常大的原始细胞可能是近四倍体白血病AML M0的一个形态学标志。
