Capasso A, Sorrentino L, Di Giannuario A, Palazzesi S, Pieretti S, Loizzo A
Department of Experimental Pharmacology, University of Naples Federico II, Italy.
Neuropsychobiology. 1994;29(3):143-51. doi: 10.1159/000119077.
The role of dexamethasone and hormones related to the hypothalamic-pituitary-adrenal (HPA) axis (corticotropin-releasing factor, adrenocorticotropic hormone, corticosterone) in the control of the spike-and-wave spindling episodes (S&W) which can be spontaneously recorded in the electrocorticogram (ECoG) of DBA/2J mice was investigated. Both dexamethasone and hormones related to the HPA axis consistently reduced the S&W in DBA/2J mice. Cycloheximide (a protein synthesis inhibitor) pretreatment significantly delayed the reducing effect of dexamethasone on the S&W in mice. After a transitory decrease, the glucocorticoid receptor antagonist RU-38486 induced a dramatic increase in the rate of the S&W episodes. In addition, mice treated with RU-38486 for 4 days showed a significant increase in the S&W 24 h after the 4th treatment. Our results indicate that dexamethasone as well as hormones related to the HPA axis are able to modulate the S&W in DBA/2J mice, suggesting that this effect may be bound to corticosteroid activity. The time lag (30-60 min) which is necessary for revealing the reducing influence of dexamethasone on the S&W in mice and the influence exerted by cycloheximide on dexamethasone activity support the hypothesis that the effect induced by dexamethasone may be at least in part dependent on genomic mechanisms.
研究了地塞米松以及与下丘脑 - 垂体 - 肾上腺（HPA）轴相关的激素（促肾上腺皮质激素释放因子、促肾上腺皮质激素、皮质酮）在控制DBA/2J小鼠脑电图（ECoG）中可自发记录的棘波 - 慢波纺锤波发作（S&W）方面的作用。地塞米松和与HPA轴相关的激素均持续降低DBA/2J小鼠的S&W。环己酰亚胺（一种蛋白质合成抑制剂）预处理显著延迟了地塞米松对小鼠S&W的降低作用。在短暂下降后，糖皮质激素受体拮抗剂RU - 38486使S&W发作率显著增加。此外，用RU - 38486处理4天的小鼠在第4次处理后24小时显示S&W显著增加。我们的结果表明，地塞米松以及与HPA轴相关的激素能够调节DBA/2J小鼠的S&W，这表明这种作用可能与皮质类固醇活性有关。揭示地塞米松对小鼠S&W的降低影响所需的时间滞后（30 - 60分钟）以及环己酰亚胺对其活性的影响支持了以下假设：地塞米松诱导的作用可能至少部分依赖于基因组机制。
