Zhou L W, Feng Y, Wang G F, Wu N H
Institute of Developmental Biology, Academia Sinica, Beijing.
Shi Yan Sheng Wu Xue Bao. 1993 Dec;26(4):463-8.
In this study, we have determined the nucleotide sequences of the cysteine tRNA gene and the original region of light-strand replication from carp (Cyprinus carpio L.) mitochondrion, which were further represented respectively in cloverleaf secondary structure and stem loop structure. By comparing the nucleotide sequences of 5 vertebrate tRNA(Cys) genes, it is found that carp mitochondrial tRNA(Cys) gene have many unusual structural features differing from those of cytoplasmic tRNA(Cys) gene. The carp mitochondrial original region of light-strand replication consists of 36 bases which include 11 base pairs in its stem and 14 bases in its loop. By making a comparison between the nucleotide sequences of the carp original region of light-strand replication and those of other 10 vertebrates, it is further found that stem sequence of carp is very conservative while loop sequence is very variable. This result indicates that stem structure may play an important role in the replication of light strand.
在本研究中，我们测定了鲤鱼（Cyprinus carpio L.）线粒体中半胱氨酸tRNA基因和轻链复制起始区域的核苷酸序列，它们分别进一步呈现为三叶草二级结构和茎环结构。通过比较5种脊椎动物tRNA（Cys）基因的核苷酸序列，发现鲤鱼线粒体tRNA（Cys）基因具有许多不同于细胞质tRNA（Cys）基因的异常结构特征。鲤鱼线粒体轻链复制起始区域由36个碱基组成，其茎中有11个碱基对，环中有14个碱基。通过比较鲤鱼轻链复制起始区域与其他10种脊椎动物的核苷酸序列，进一步发现鲤鱼的茎序列非常保守，而环序列非常可变。这一结果表明茎结构可能在轻链复制中起重要作用。
