结直肠类癌的流式细胞术DNA分析

Flow cytometric DNA analysis of colorectal carcinoid.

作者信息

Cheng J Y, Lin J C, Yu D S, Lee W H, Meng C L

机构信息

Department of Surgery, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.

出版信息

Am J Surg. 1994 Jul;168(1):29-32. doi: 10.1016/s0002-9610(05)80066-2.

DOI:10.1016/s0002-9610(05)80066-2

PMID:8024095

Abstract

We analyzed the patterns of DNA ploidy in 31 colorectal carcinoid tumors from paraffin-embedded tissues by DNA flow cytometry and the relationship of the patterns of DNA ploidy to prognosis. Diploid DNA was found in 78% (24 of 31) of carcinoids and tetraploid in 6% (2 of 31). Five (16%) carcinoids were DNA aneuploid, and four of the patients with aneuploidy showing a near-hypertriploid pattern died during the first 5 years of follow-up. The association of aneuploidy with stage, size, and invasion of tumor was significant. However, our data indicated that DNA aneuploidy of a near-hypertriploid pattern was the most precise and reliable parameter for predicting the prognosis of colorectal carcinoid tumors.

摘要

我们通过DNA流式细胞术分析了31例石蜡包埋组织中的大肠类癌肿瘤的DNA倍体模式，以及DNA倍体模式与预后的关系。78%（31例中的24例）类癌为二倍体DNA，6%（31例中的2例）为四倍体。5例（16%）类癌为DNA非整倍体，其中4例非整倍体呈近超三倍体模式的患者在随访的前5年内死亡。非整倍体与肿瘤分期、大小和浸润之间的关联具有显著性。然而，我们的数据表明，近超三倍体模式的DNA非整倍体是预测大肠类癌肿瘤预后最精确和可靠的参数。