Wellemans J, Cerny R L, Gross M L
Department of Chemistry, University of Nebraska-Lincoln 68588-0304.
Analyst. 1994 Apr;119(4):497-503. doi: 10.1039/an9941900497.
Tandem mass spectrometry (MS-MS) has proved to be a state-of-the-art technique for the structure of synthetic and biological compounds. One opportunity for MS-MS is the study of modified deoxyribonucleic acid (DNA) bases resulting from the attachment of carcinogens such as polycyclic aromatic hydrocarbons (PAHs). The determination of PAH-DNA adducts, formed in vivo via a one-electron oxidation or a diol-epoxide mechanism, requires high efficiency separation and very sensitive techniques. This is because the analyte will occur in complex biological mixtures and at low femtomole levels, considering that one modification occurs for 10(6) or 10(8) bases. This paper reviews various approaches to the separation and mass spectrometric structural determination of DNA adducts. The main emphasis of our research is the sub-picomolar detection and identification of DNA-PAH adducts, particularly those formed via a one-electron oxidation mechanism.
串联质谱法（MS-MS）已被证明是一种用于分析合成化合物和生物化合物结构的先进技术。MS-MS的一个应用领域是研究由多环芳烃（PAH）等致癌物附着而产生的修饰脱氧核糖核酸（DNA）碱基。通过单电子氧化或二醇环氧化物机制在体内形成的PAH-DNA加合物的测定，需要高效分离和非常灵敏的技术。这是因为考虑到每10⁶或10⁸个碱基中才会出现一次修饰，分析物会存在于复杂的生物混合物中，且含量处于低飞摩尔水平。本文综述了DNA加合物的分离及质谱结构测定的各种方法。我们研究的主要重点是亚皮摩尔级的DNA-PAH加合物检测与鉴定，特别是那些通过单电子氧化机制形成的加合物。
