Sine J P, Toutant J P, Colas B
Laboratoire de Biochimie, Centre de Recherche de Biologie et Physicochimie Cellulaires, Faculté des Sciences et des Techniques, Nantes, France.
Biochem Biophys Res Commun. 1994 Jun 30;201(3):1376-81. doi: 10.1006/bbrc.1994.1855.
Mucosal cells of rat intestine express amphiphilic monomer (G1) and dimer (G2) as well as hydrophilic tetramer (G4) of butyrylcholinesterase (BChE). After incubation with heparin (3 and 15 microM), amphiphilic G2 form showed decreased migrations in nondenaturing electrophoresis whereas in these conditions, the mobility of hydrophilic forms from rat and human serum BChEs was unchanged. Sucrose gradient sedimentation and chromatography performed on HPLC gel filtration and on heparin-Sepharose confirmed that amphiphilic G1 and G2 forms were able to bind heparin. Increasing concentrations of heparin resulted in higher sizes of heparin-BChE complex. These properties of intestinal BChE may be related to the possible occurrence of endogenous heparin in this tissue.
大鼠肠道的黏膜细胞表达丁酰胆碱酯酶（BChE）的两亲性单体（G1）和二聚体（G2）以及亲水性四聚体（G4）。在用肝素（3和15微摩尔）孵育后，两亲性G2形式在非变性电泳中迁移率降低，而在这些条件下，大鼠和人血清BChE的亲水性形式的迁移率未发生变化。在HPLC凝胶过滤和肝素-琼脂糖上进行的蔗糖梯度沉降和色谱分析证实，两亲性G1和G2形式能够结合肝素。肝素浓度的增加导致肝素-BChE复合物的尺寸增大。肠道BChE的这些特性可能与该组织中内源性肝素的可能存在有关。
