促肾上腺皮质激素释放因子对离体灌注大鼠心脏心房利钠肽释放的影响。

Effect of corticotropin releasing factor on atrial natriuretic peptide release from the isolated perfused rat heart.

作者信息

Haug C, Grunt M, Schmid S, Steinbach G, Metzele A, Maier V, Keck F S, Grünert A

机构信息

Institute of Clinical Chemistry, University of Ulm, Fed. Rep. of Germany.

出版信息

Arzneimittelforschung. 1994 May;44(5):579-82.

PMID:8024626

Abstract

The effect of corticotropin releasing factor (CRF) on atrial natriuretic peptide (ANP) release and its possible modulation by indomethacin, norepinephrine, propranolol and nitro-L-arginine (an inhibitor of the endothelium-derived relaxing factor (EDRF) release) was investigated, using an isolated perfused rat heart preparation. Bolus injection of 5 micrograms CRF, dissolved in 100 microliters perfusion buffer, provoked a significant (p < 0.01 vs. control) short-time increase of ANP release. Indomethacin (3 x 10(-5) mol/l) inhibited the CRF-stimulated increase of ANP release and decreased the basal ANP secretion (p < 0.01 vs. CRF group). Norepinephrine (10(-9) mol/l) slightly, but not significantly, decreased the CRF-stimulated ANP release and did not change the basal ANP output. Propranolol (3 x 10(-6) mol/l) did not alter ANP release. Nitro-L-arginine (3 x 10(-5) mol/l) increased the basal ANP release (p < 0.01 vs. CRF group) and prolonged the CRF-induced rise of the ANP secretion. The present data suggest that prostaglandins are important mediators of basal and CRF-stimulated ANP release and that EDRF might be a physiological inhibitor of ANP release.

摘要

采用离体灌注大鼠心脏标本，研究促肾上腺皮质激素释放因子（CRF）对心房利钠肽（ANP）释放的影响，以及吲哚美辛、去甲肾上腺素、普萘洛尔和硝基-L-精氨酸（一种内皮源性舒张因子（EDRF）释放抑制剂）对其的可能调节作用。将5微克CRF溶解于100微升灌注缓冲液中进行单次推注，可引起ANP释放显著（与对照组相比，p<0.01）短期增加。吲哚美辛（3×10⁻⁵摩尔/升）抑制CRF刺激的ANP释放增加，并降低基础ANP分泌（与CRF组相比，p<0.01）。去甲肾上腺素（10⁻⁹摩尔/升）轻微但不显著地降低CRF刺激的ANP释放，且不改变基础ANP输出。普萘洛尔（3×10⁻⁶摩尔/升）不改变ANP释放。硝基-L-精氨酸（3×10⁻⁵摩尔/升）增加基础ANP释放（与CRF组相比，p<0.01），并延长CRF诱导的ANP分泌升高。目前的数据表明，前列腺素是基础和CRF刺激的ANP释放的重要介质，且EDRF可能是ANP释放的生理抑制剂。