Deng D J, Mueller K, Kasper P, Mueller L
Beijing Institute for Cancer Research, China.
Biomed Environ Sci. 1994 Mar;7(1):85-90.
Most of anticancer drugs are mutagenic. A possible exception is diallyl trisulfide (DAT), a component of garlic. It is an antimutagenic anticancer chemical although it is mainly used as antibiotic. Its modifying effect on induction of UDS by mutagenic mitomycin C (MMC), cyclophosphamide (CP) and cis-diamine dichloroplatin (DDP) was investigated with the UDS assay in the primary cultures of Wistar rat hepatocytes (hpc) using the autoradiographic technique. Results showed that 1.0-4.0 nmol/ml of DAT did not induce UDS and that MMC, CP and DDP resulted in a significant induction of dose-dependent UDS. DAT enhanced induction of UDS by these drugs. A dose-effect relationship was observed between dose of DAT and enhancement of induction of UDS. However, the mechanism of the enhancement is not clear.
大多数抗癌药物具有致突变性。大蒜的成分二烯丙基三硫化物（DAT）可能是个例外。它是一种抗诱变的抗癌化学物质，尽管它主要用作抗生素。利用放射自显影技术，通过Wistar大鼠肝细胞原代培养物中的UDS测定法，研究了其对诱变剂丝裂霉素C（MMC）、环磷酰胺（CP）和顺二氯二氨铂（DDP）诱导UDS的修饰作用。结果表明，1.0 - 4.0 nmol/ml的DAT不诱导UDS，而MMC、CP和DDP可显著诱导剂量依赖性UDS。DAT增强了这些药物对UDS的诱导作用。在DAT剂量与UDS诱导增强之间观察到剂量效应关系。然而，增强机制尚不清楚。
