Hamaguchi S, Ogata H, Masawa N
First Department of Anesthesiology, Dokkyo University School of Medicine, Tochigi, Japan.
No To Shinkei. 1994 Apr;46(4):355-9.
This experiment was performed to investigate whether hypertonic saline has a preventive effect on delayed neuronal death in the CA1 subfield of the hippocampus. Twenty gerbils were used, and after being anesthetized by inhalation of 1% halothane, both common carotid arteries were occluded for 2.5 min. The animals were injected intravenously with 2 ml/kg of 10% NaCl immediately after reperfusion, and 2 ml/kg of physiological saline solution was used in the same manner in a control group. Five days later, histopathological changes in the CA1 subfield were observed by staining with hematoxylin-eosin and examining sections of the brain under a light microscope. Degenerative or necrotic pyramidal cells exhibited cell shrinkage, nuclear pyknosis, dark staining of the cytoplasm vacuolation and disappearance of the radial striated zone. The pyramidal cell degeneration rate in a 1 mm length of CA1 subfield was 95.6 +/- 1.6% in the ischemia-reperfusion-saline group and 7.1 +/- 3.0% in ischemia-reperfusion-hypertonic saline group, and the difference was statistically significant. This study verified that hypertonic saline prevented delayed neuronal death in the CA1 subfield of hippocampal area after ischemia-reperfusion.
本实验旨在研究高渗盐水对海马CA1亚区迟发性神经元死亡是否具有预防作用。选用20只沙鼠，吸入1%氟烷麻醉后，双侧颈总动脉闭塞2.5分钟。再灌注后立即静脉注射2ml/kg的10%氯化钠，对照组以同样方式注射2ml/kg的生理盐水。五天后，通过苏木精-伊红染色并在光学显微镜下检查脑切片，观察CA1亚区的组织病理学变化。变性或坏死的锥体细胞表现为细胞皱缩、核固缩、细胞质空泡化染色加深以及放射状条纹区消失。缺血再灌注生理盐水组CA1亚区1mm长度内的锥体细胞退变率为95.6±1.6%，缺血再灌注高渗盐水组为7.1±3.0%，差异具有统计学意义。本研究证实，高渗盐水可预防缺血再灌注后海马区CA1亚区的迟发性神经元死亡。
